Postersession III
Onsdag den 23. oktober
11:00 – 12:00
lokale: Helsinki/Oslo
Chairmen: Peter Holmberg Jørgensen / Stig Brorson Hansen
176. Tendon and skeletal muscle matrix gene expression and functional responses to immobilization and rehabilitation in young males: Effect of growth hormone administration
Anders Ploug Boesen, Kasper Dideriksen, Peter Schjerling, Christian Couppe, Michael Kjaer, Henning Langberg
Deptartment Ortopaedic Surgery, Institute of Sports Medicine Copenhagen, Bispebjerg Hospital and Center for Healthy Aging, Faculty ; Department of Public Health, Faculty of Health and Medical Sciences , CopenRehab, University of Copenhagen, Denmark
Background: Counteracting the loss of muscle and
tendon function during periods of
immobilization and recovery of this
during rehabilitation represents a
challenge in clinical medicine
Purpose / Aim of Study: The aim of the study was to examine the
effect of growth hormone (GH) on
connective tissue of tendon and skeletal
muscle during immobilization and
retraining in humans
Materials and Methods: Young men (20-30 years; n=20) were
randomly assigned to daily recombinant
GH (rhGH) (33-50µg/kg/d) or placebo
(Plc), and had one leg immobilized for
two weeks followed by six weeks of
strength training. Cross sectional area
(CSA), maximal isometric muscle
strength (MVC) and biomechanical
properties of m.quadriceps and patellar
tendon were determined. Muscle and
tendon biopsies were analysed for
mRNA of collagen (COL 1A1/3A1),
insulin-like growth factors (IGF-1Ea/Ec)
and lysyloxidase (LOX).
Findings / Results: In skeletal muscle the CSA and MVC
declined with immobilization, and
recovered with rehabilitation similar in
both groups. Likewise, both groups
increased in IGF-1Ea/Ec and COL
1A1/3A1 expression in muscle during
retraining after immobilization compared
to baseline, and the rise was more
pronounced when subjects received
rhGH (p<0.05). The tendon CSA did not
change during immobilization, but
increased in both groups during six
weeks of rehabilitation (GH: +17%, Plc:
+11, p<0.05). A decline in tendon
stiffness after immobilization was
observed only in Plc (p<0.05), and an
increase during six weeks rehabilitation
was observed only in GH (p<0.05). IGF-
1Ea and COL 1A1/3A1 mRNA increased
with immobilization in the GH group only
(p<0.05), and LOX mRNA was after
immobilization higher in the GH group vs.
Plc (p<0.05).
Conclusions: In conclusion, GH stimulates collagen
expression in both skeletal muscle and
tendon and abolishes the normal
inactivity related decline in tendon
stiffness and in tendon LOX, and results
in an increased tendon CSA and
stiffness during rehabilitation. GH has a
matrix stabilizing effect during periods
with inactivity and rehabilitation in
humans.
181. Comparison of Two Humeral Head Resurfacing Implants. 2 year Results of a Randomized Controlled Clinical Trial
Inger Mechlenburg, Thomas Klebe, Kaj Døssing, Kjeld Søballe, Maiken Stilling
Orthopaedic Research, Aarhus University Hospital; Orthopaedic Department, Regional Hospital Silkeborg
Background: Humeral head resurfacing implants
(HHRI) are used to preserve bone
stock and restore normal anatomy in
the osteoarthritic shoulder joint.
Purpose / Aim of Study: To examine the clinical results, implant
migration and bone density changes in
proximity of the Copeland (Biomet Inc.)
and the Global C.A.P. (DePuy Int)
HHRI.
Materials and Methods: 31 patients (12 females) mean age of
63 (39-82) years with shoulder
osteoarthritis were randomly allocated
to a Copeland (n=13) or Global C.A.P.
implant (n=18). Post-op, 6, 12, 24
weeks 1 and 2 years migration of the
implants was measured with
radiostereometry (RSA). Bone mineral
density (BMD) was measured with
DXA. Pre-op, 3,6,12 and 24 months
Constant Shoulder Score (CSS) and
Western Ontario Osteoarthritis of the
Shoulder Index (WOOS) were
recorded.
Findings / Results: 2 patients were lost to follow-up and 5
implants were revised (3 Copeland, 2
Global C.A.P.)
At 2 years total translation (TT) was
0.60mm (SD 0.41) for the Copeland
and 1.01mm (SD 0.66) for the Global
C.A.P. (p=0.18). Between 1 and 2
years TT for the Global C.A.P.
increased (p=0.02) whereas the
Copeland HHRI had no significant
migration (p=0.15).
At 6 months BMD had decreased from
0.59 to 0.42 g/cm2 (p=0.01) around the
Copeland implant and from 0.44 to
0.37 g/cm2 (p=0.21) around the Global
C.A.P. No differences between groups.
Copeland: CSS increased from 57, 61,
71, 72, 77 and WOOS improved from
939, 645, 296, 295, 113. Global C.A.P:
CSS increased from 35, 51, 65, 73, 73
and WOSS improved from 1088, 568,
383, 381, 300. Both groups improved
significantly over time (p<0.01) with no
differences between the groups.
Conclusions: No difference in TT between the
groups. Bone was lost in proximity of
both implants. Patient-assessed
scores improved comparably in both
Groups.
182. Clinical implications of positive cultures in revision shoulder arthroplasty
Thomas Falstie-Jensen, Janne Ovesen, Viggo S. Johannsen
Orthopaedic, Aarhus University Hospital
Background: During the recent years
proprionibacterium acne is reported to
be an common pathogen in shoulder surgery.
We reviewed our revisions to judge if
any change in diagnostic approach was
needed
Purpose / Aim of Study: The aim of the study was to
retrospectively evaluate the prevalence
of positive cultures obtained at
revision shoulder arthroplasty.
Materials and Methods: From January 2009 to December 2012 we
revised 104 shoulder arthroplasties
because of pain, stiffness and
functional limitations. In 69 patients
biopsies were cultured either because of
suspected deep infection or as a routine
precaution.
All results from cultures and
preoperative blood samples were recorded.
Findings / Results: Cultures were positive in 31 out of the
69 sample sets. The most frequent
pathogen was Proprionibacterium Acnes,
which was identified in 19 of the
31sets. In the remaining cultures a
variety of pathogens were found,
primarily of low virulence
The main symptoms for patients with
positive cultures especially of the
Proprionibacterium subgroup were pain
and stiffness and very rarely the
classical signs of infection. Blood
samples displayed normal CPR, ESR and
leucocyte counts in many cases
During revision surgery membrane
formation around the prosthetic
components, severe stiffness, cloudy
fluid and osteolysis were associated
with an increased likelihood of infection.
Conclusions: Proprionibacterium Acnes is a very
common pathogen in revision shoulder
arthroplasty and should be suspected in
painful and stiff shoulders. Suspicion
of deep infection is of clinical
importance in decision making regarding
prosthesis retention or removal and
close collaboration with a microbiology
department is important in detecting and
treating these infections. . Selection
of appropriate antibiotics treatment
should take into consideration that
propionibacteriae are among the most
common pathogens.
184. Construct validity and responsiveness of functional measures used in subjects following an outpatient prosthetic rehabilitation program after a major lower limb amputation
Kajsa Lindberg, Joanne Boelskifte, Jimmy Johansson, Mie Rinaldo, Morten Tange Kristensen
Rehabilitation Center Vanløse, Community of Copenhagen; Physical Medicine and Rehabilitation Research-Copenhagen (PMR-C), Department of Physical Therapy and, Copenhagen University Hospital Hvidovre
Background: Subjects surviving a major lower limb
amputation (LLA) are quite often
provided with prosthesis for optimised
function in daily life, but there is a lack
of knowledge about the effect of
rehabilitation programs, as to which
tests to be used to show progress.
Purpose / Aim of Study: To examine construct validity and
internal responsiveness of
performance-based measures of
functional mobility and endurance after
LLA.
Materials and Methods: Eighteen subjects with a recent below
knee amputation (BKA), and 15 with
an above knee amputation (AKA), 6
women and 27 men with a mean age
of 63.6 (SD, 12.4). All subjects
followed a rehabilitation program (twice
weekly), focused at improving balance,
strength and prosthetic function, and
performed the Timed Up and Go
(TUG), the 10 meter fast speed
walking test (10MWT) and 2 minute
walking test (2MWT) at baseline and at
end of the program. Pearson´s r
assessed construct validity, while
internal responsiveness was assessed
by calculating the effect size I (ESI) as
the mean change in performance
scores / by the baseline SD, and effect
size II (ESII) as the mean change in
scores / by the SD of changes.
Findings / Results: The three outcome measures were
highly correlated (r>0.66, P<0.001)
after the program, while effect size was
high to medium; 10MWT (ESI 1.03,
ESII 1.06), 2MWT (ESI 1.0, ESII 1.15)
and TUG (ESI 0.63, ESII 0.94).
Performances improved from 39-99%
and subjects with a BKA performed all
tests significantly faster (P=0.01) than
those with an AKA. Eleven subjects
walked 1.0 m/s or faster at end of
training.
Conclusions: Construct validity and internal
responsiveness of the three measures
were high, and is recommended be
used by other centres. Still, only one
third walked faster than 1m/s,
supporting the need for studies
examining the effect of e.g.
progressive strength training for
improvements.
185. Pneumococcal sepsis-induced Symmetrical Peripheral Gangrene
Taj Haubuf, Klaus Kjær Petersen , Kurt Fuursted, Alex Lund Laursen, Johnny Keller
Orthopedic, Aarhus University Hospital; Microbiology, Aarhus University Hospital; Infectious Disease , Aarhus University Hospital
Background: Symmetrical Peripheral Gangrene -
SPG (also known as Purpura
Fulminans) caused by Streptococcus
pneumonia (SP) is an uncommon,
though very severe and potential lethal
complication to septicemia.
Purpose / Aim of Study: To describe our experience with
patients with SPG.
Materials and Methods: Between 2003 and 2013 eleven
patients SPG due to SP where treated
at Aarhus University Hospital. Patient’s
files were retrospectively studied
regarding clinical status,
predispositions, multi-organ
involvement, days of admission,
surgical treatment and prostheses
status.
Findings / Results: Eleven patients, median age 55 years
(range 38-71 years) admitted with
septicemia developed SPG. Four
patients had earlier undergone
splenectomy and one patient had no
spleen, due to SLE. Six were smokers
and only one patient had no obvious
predisposing conditions. SP sensitive
to penicillin was found in blood
samples in all patients. All had purpura
and eight had skin necrosis of lips and
nose. All patients received high doses
of penicillin and intensive care, three
patients died during hospitalization,
one prior to surgical intervention. Ten
patients required extensive surgical
intervention resulting in four humeral-,
four antebrachium-, 28 digits and hand-
, four femoral-, three through knee-,
eleven crural and one toe amputations.
Nine patients underwent dialysis and
seven received vasopressors. Median
time from onset of symptoms to
amputation was 21,7 days (range 5-
100 days). After a protracted
rehabilitation period six patients were
mobilized on bilateral leg prostheses.
One patient became wheelchair bound.
Conclusions: Symmetrical Peripheral gangrene is a
serious complication to Streptococcus
pneumonia septicaemia with at high
mortality- and complication rate with
risk of extensive amputations, resulting
in long admissions and loss of daily
functions.
186. Ultrasound guided core needle biopsy of peripheral nerve sheath tumors. A retrospective study.
Damgaard Jacob , Hauge Hansen Bjarne , Holmberg Jørgensen Peter , Keller Johnny, Hellfritzch Michel , Baad-Hansen Thomas
Center for Bone and Soft Tissue Sarcomas, Aarhus University Hospital, Denmark; Dept. of Radiology, Aarhus University Hospital, Denmark
Background: Surgical biopsies have until recently been
the preferred method for diagnosis of tumors
arising form peripheral nerve tissue.
Ultrasound guided core needle biopsy
(UGCNB) has within the last few years been
introduced in diagnosis of these tumours.
Fewer complications, less unpleasantness
for the patients, faster recovery, and quicker
diagnostic work-up have been reported.
Purpose / Aim of Study: The aims of the study were to identify
causes of UGCNB complications, to
evaluate diagnostic strength of UGCNB in
terms of grading between benign and
malignant specimens, and finally, to describe
time consumption using this diagnostic
method.
Materials and Methods: We retrospectively reviewed 69 patients
who underwent UGCNB between January
2004 and December 2012. Complications
due to the procedure were assessed.
Sensitivity, specificity; positive predictive
value (PPV) and negative predictive value
(NPV) were calculated based on the current
dataset. Furthermore, time from referral to
final diagnosis was calculated.
Findings / Results: A single patient described mild pain after the
procedure, which resolved after 2 days. No
permanent injuries were reported. In 82% of
the cases, UGCNB was able to distinguish
between malignant and benign material.
Sensitivity was 0.8, specificity was 1.0, PPV
was 1.0, and NPV was 0.97. Mean time from
referral to UGCNB was 7 days (range 0-43
days). Mean time from UGCNB until the final
diagnosis was 7 days (range 1-21 days).
Conclusions: UGCNB of PNST is a safe method.
However, UGCNB cannot stand alone in the
clinical work-up due to the risk of false
negative results. The results shows, that
time from referral to decision of treatment is
acceptable and in accordance with the
National Danish Cancer Patient Pathway for
soft tissue sarcomas.
187. Local Recurrence Rate after Surgical Excision of Desmoid Fibromatosis.
Elinborg Mortensen, Thea Hovgaard, Michael Mørk Petersen
Department of Orthopaedic Surgery, Rigshospitalet, Denmark
Background: Surgical treatment of desmoid
fibromatosis is still the standard
treatment but oncologic treatment with
medicine or radiation therapy is
increasingly applied.
Purpose / Aim of Study: To determine the local recurrence rate
among surgically excised desmoid
fibromatosis.
Materials and Methods: We retrospectively assessed all
tumours (34 patients (F/M = 25/9,
mean age 38 (12 – 67) years)) of the
extremities, spine and trunk wall
(abdominal wall/ other
locations=13/21) with the histology
desmoid fibromatosis surgically
excised at our clinic between 1995 and
2005. Patients primarily operated on in
other clinics or operated on for a local
recurrence were excluded. Data were
extracted from the patient files and
from the Danish National Pathology
Registry (DNPR). Statistics: Data are
presented as mean (range). Kaplan
Meir survival analysis.
Findings / Results: Mean tumor size was 6.1 (2-15) cm
and the surgical margins obtained
were: wide (n=28), intralesional (n=3)
or inconclusive (n=3). 7 patients had a
local recurrence 21.5 (5-51) months
after initial tumour excision (mean
DNPR follow-up for patients without
recurrences was 119 (96-220) months)
corresponding to a probability of 5-year
local recurrence free survival of 79%.
Local recurrences were seen in 6
patients with wide margin and 1 patient
with intralesional margin. The majority
of local recurrences were seen in
patients with tumours of the extremities
and paraspinal location (n=5) and 2
abdominal wall tumour recurred.
Conclusions: Overall the treatment seemed
reasonable with a 79% probability
avoiding local recurrences after 5
years. The recurrences did not seem
to depend on the surgical margin, thus
a less aggressive approach not always
aiming at wide tumour resection could
be considered.
188. Persistent wound drainage after tumor resection and endoprosthetic reconstruction the proximal femur
Peter Horstmann, Werner Hettwer, Tomas Grum-Schwensen, Michael Mørk Petersen
Department of Orthopaedic Surgery, Department of Orthopaedic Surgery, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denma
Background: Persistent post-operative wound drainage is associated with an increased incidence of periprosthetic infection and occurs in about 4% of conventional total hip arthroplasties. Similar data is not available for endoprosthetic reconstruction of the proximal femur after tumor resection.
Purpose / Aim of Study: To determine the duration of postoperative surgical wound drainage, administration of antibiotics and date of discharge, and to establish if a change in the wound closure routine could improve these variables.
Materials and Methods: We performed a retrospective review of all adult patients (n=41, mean age = 63 (37-86) years) who received a proximal femoral tumor arthroplasty in our department in 2012 and collected similar data prospectively from 2013 (n=5, mean age = 72 (54-86) years), where routine wound closure with staples was substituted with intradermal suture, application of Steristrips and an occlusive skin adhesive (Liquiband Flex).
Findings / Results: In 2012 mean duration of post operative wound drainage was 8 (2-45) days, mean duration of administration of postoperative antibiotics was 8.2 (1-45) days and mean hospital stay was 9.4 (3-45) days. Nineteen (45%) had prolonged wound drainage (7 days or longer). The preliminary first 5 patients, who underwent skin closure with a skin adhesive, all had dry wounds at the first scheduled post operative dressing change (mean 2.9 (2-4) days), mean duration of post operative administration of antibiotics was reduced to 3.6 (2-7) days and mean hospital stay was reduced to mean 6.5 (3-10) days.
Conclusions: Our small sample showed a high prevalence of prolonged drainage from the surgical site. A simple change in the wound closure routine appears to show a promising reduction in wound drainage, postoperative antibiotic administration, and hospital stay. Further studies are warranted.
190. Testing A New Type Of Osteosynthesis And After Care – In Treatment Of Antebrachium Fractures in Children, Preliminary Result Of A Prospectiv Case-Control Study.
Ture Karbo, Stig Sonne-Holm, Christian Wong
Pediatric orthopaedic section, Hvidovre University Hospital
Background: Fractures of the lower arm in children are
the most common, comprising about
40% of all pediatric fractures. Generally
there is consensus regarding treatment,
but the displaced distal dia-metaphyseal
fractures (DDMF) are there no apparent
adequate surgical option.
A new type of combined internal elastic
nail and external fiksation (MIROS@)
was applied in 10 patients compared with
10, age and fracture type matched,
children.
Purpose / Aim of Study: To compare MIROS@ to the conventional
osteosynthesis methods in treatment of
children with DDMF of the antebrachium.
Materials and Methods: The prospective case-control study
including 20 children, age 4-15, operated on
with standard treatment - with elastic nails /
Kirschner wires or MIROS@ for DDMF.
Follow-up at 3 month after removal of
osteosynthesis material including x-rays of
both antebrachii, systematic testing of
bilateral range of motion, visual analogue
pain scale and strength measurements.
Findings / Results: There were minor differences in clinical and
radiological outcome after 3 month follow up.
Operating for insertion and removal time of
the MIROS was faster with a smaller
surgical incision.
All Miros@ had a plaster-cast-free
aftercare.
Conclusions: MIROS@ is as good as conventional
osteosynthesis methods at 3 month follow-
up, though having advantages – faster
operating time, less surgical scaring, faster
removal, no plaster cast and early
movement of elbow-/wrist-joints.
However, prospective randomized trials
should be performed.
191. Normal distribution of seating balance for healthy Danish children
Line Kjeldgaard Pedersen, Ahmed Abdul-Hussein Abood, Ole Rahbek, Bjarne Møller-Madsen
Department of Children’s Orthopedics, Aarhus University Hospital
Background: Measurement of seating balance is a
promising method for analysis and
evaluation of children’s seating
balance in relation to orthopedic
conditions such as hip dislocation and
scoliosis. Furthermore seating balance
can be used to evaluate postural
control in children with
neurodevelopmental diseases such as
Cerebral Palsy.
Purpose / Aim of Study: To develop a normal distribution of
seating balance in healthy Danish
Children for comparison with children
with orthopedic disorders.
Materials and Methods: Sixty-six children aged 7-14 years from
the 1th, 3 th, 5th and 7th levels were
included at a Danish primary school
after informed consent were obtained.
Tekscan CONFORMat Research was
used and the measurements of seating
position were analyzed with the
Tekscan Sway Analysis Module. Three
consecutive measurements of both
normal seating and seating in up-right
position were used to calculate the
average result for each of the 5
parameters of balance. The
percentage of pressure distribution on
the left and right side were calculated.
Findings / Results: Data were stratified according to age,
weight and height. For the 1th level
(n=15) two of five parameters for
balance showed improvement in the
up-right position (p˂0,0003, p˂0,013)
and primarily for females. Furthermore
a significant correlation was found
between seated balance and both
height and weight. Statistical analysis
with STATA 11 was used, and
Spearman correlation and students t-
test was calculated.
Conclusions: We measured the effect of BMI, age,
gender and back position on seating
balance and have established a
normal distribution for seating balance
in healthy Danish children. This normal
distribution can be used as reference
data for comparison of seating balance
in children with orthopedic disorders in
a clinical setting.
192. Hamstring lengthening in CP patients by needle tenotomy is safe
Lauge Østergaard, Gert Rahbek Andersen
Orthopedic Department U, Rigshospitalet
Background: Crouch gait among CP patients are caused
by hip and knee flexion contractures.
Crouch gait can be treated by lengthening of
the hamstrings. Hamstring lengthening is
usually done by open surgery.
Purpose / Aim of Study: This study examines whether there is any
statistical significant difference between
making hamstring lengthening by open
surgery or by hypodermic needle tenotomy.
Materials and Methods: Data were collected retrospectively by
looking through medical records. 59 patients
(93 limbs) were included in this study and all
the patients had undergone hamstring
lengthening. The population was subdivided
into two groups. Group 1 (44 patients, 65
limbs) had their hamstring lengthened by
tenotomy with a hypodermic needle. Group
2 (15 patients, 28 limbs) had their hamstring
lengthened by open surgical procedure.
Findings / Results: Of the 44 patients in group 1 there was one
adverse effect. This patient had pain
postoperatively in the lateral hamstring on
the operated leg. Of the 15 patients in group
2 there was one adverse effect as well. This
patient experienced pain around the area of
the ischiadic tubercle. In group 1 there was
one relapse in the follow up period and in
four situations the surgical procedure had no
effect. In group 2 there was no effect of the
surgical procedure in two cases.
Conclusions: There was no statistical significant
difference (0,5>p>0,25) between the rate of
complications in the two groups.
Furthermore there was no statistical
difference on the missing effect or relapse of
the surgical procedure between the two
groups.
Hamstring lenthening by hypodermic nedle
tenotomy is safe and outcome is
comparable to open surgery; is easy to
perform in supine position and thus offers
perioperative evaluation of the effectiveness
of the lenthening.